{"id":4825,"date":"2020-11-10T14:33:54","date_gmt":"2020-11-10T22:33:54","guid":{"rendered":"https:\/\/stagewp.westernu.edu\/osteopathic\/?page_id=4825"},"modified":"2023-10-16T14:45:03","modified_gmt":"2023-10-16T21:45:03","slug":"szurmant-lab","status":"publish","type":"page","link":"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/szurmant-lab\/","title":{"rendered":"Szurmant Lab"},"content":{"rendered":"<section class=\"hero-secondary\">\n  <div class=\"relative bg-black\">\n                <img decoding=\"async\" class=\"adhere object-cover\" src=\"\/media\/osteopathic\/szurmant.jpg\" srcset=\"\" sizes=\"100vw\" alt=\"szurmant image\">\n      <div class=\"adhere gradient-overlay\"><\/div>\n        <div class=\"relative container pt-56 md:pt-112 pb-144 md:pb-192\">\n      <div class=\"md:flex md:justify-between px-12\">\n        <div class=\"sm:w-8\/12 md:w-6\/12 xl:w-5\/12 px-12\">\n                      <div class=\"mb-20\">\n              <div class=\"block font-sans font-bold text-14 text-white leading-130\">\n  <span class=\"inline-block\">\n    <a href=\"\/\" class=\"block hover:underline\">WesternU<\/a>\n  <\/span>\n      <span class=\"inline-block mx-6\">\/<\/span>\n          <a href=\"https:\/\/stagewp.westernu.edu\/osteopathic\" class=\"inline-block hover:underline\">College of Osteopathic Medicine of the Pacific (COMP) and COMP-Northwest<\/a>\n          <span class=\"inline-block mx-6\">\/<\/span>\n      <a href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/\" class=\"inline-block hover:underline\">Research<\/a>\n    <span class=\"inline-block mx-6\">\/<\/span>\n      <a href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/\" class=\"inline-block hover:underline\">Research Labs<\/a>\n    <span class=\"inline-block mx-6\">\/<\/span>\n    <span class=\"inline-block\">COMP (CA): Szurmant Lab<\/span>\n  <\/div>            <\/div>\n                                        <h1 class=\"t-h1-sans  text-white\">COMP (CA): Szurmant Lab<\/h1>\n        <\/div>\n              <\/div>\n    <\/div>\n  <\/div>\n<\/section>\n\n\n<section class=\"relative my-48 md:my-72\">\n  <div class=\"container\">\n    <div class=\"lg:flex px-12 lg:justify-start\">\n              <div class=\"w-full lg:w-5\/12 2xl:w-4\/12 -mt-80 md:-mt-112 mb-48 px-12 lg:pr-32\">\n                              <nav class=\"py-28 px-40 2xl:px-64 rounded-5 md:rounded-8 bg-gray-darker text-white mb-40\">\n            <ul>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/\" target=\"_self\" rel=\"noopener\">Research Labs<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/bi-lab\/\" target=\"_self\" rel=\"noopener\">Bi Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/elsalanty-lab\/\" target=\"_self\" rel=\"noopener\">Elsalanty Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/fricano-lab\/\" target=\"_self\" rel=\"noopener\">Fricano Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/nalley-lab\/\" target=\"_self\" rel=\"noopener\">Nalley Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/peterfy-lab\/\" target=\"_self\" rel=\"noopener\">Peterfy Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/scott-lab\/\" target=\"_self\" rel=\"noopener\">Scott Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/sperling-lab\/\" target=\"_self\" rel=\"noopener\">Sperling Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/steinauer-lab\/\" target=\"_self\" rel=\"noopener\">Steinauer Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/szurmant-lab\/\" target=\"_self\" rel=\"noopener\">Szurmant Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/venketaraman-lab\/\" target=\"_self\" rel=\"noopener\">Venketaraman Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/wagner\/\" target=\"_self\" rel=\"noopener\">Wagner Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/wedel-lab\/\" target=\"_self\" rel=\"noopener\">Wedel Lab<\/a>\n                <\/li>\n                          <\/ul>\n          <\/nav>\n                            <\/div>\n      \n      \n                  \n                  \n      <div class=\"w-full px-24 wysiwyg-content lg:w-7\/12 2xl:w-7\/12\">\n        <h2>Bacterial signal transduction systems and their role in physiology, antimicrobial resistance and utility as drug targets<\/h2>\n<p>Pathogenic bacteria are some of the most formidable threats to human health. These threats appeared all but eliminated, thanks due to the discovery of powerful antibiotics. The constant exposure of bacteria to these antibiotics has selected for potent multi-drug resistant bacteria, so-called superbugs that are making a strong comeback. To cope with this renewed threat a dedicated effort by the scientific community is needed to identify new drug targets and to generate inhibitors of such targets. The Szurmant laboratory contributes to this endeavor by studying essential aspects of bacterial physiology and signal transduction in model bacteria and selected pathogens. A unique feature of the lab is the integration of information stemming from numerous disciplines, including structural biology, genetics, molecular biology, bioinformatics and biophysics.<\/p>\n\n      <\/div>\n    <\/div>\n  <\/div>\n<\/section>\n\n\n\n\n\n\n\n<section class=\"relative my-48 md:my-72\" vue-app>\n  <div class=\"container\">\n        <app-accordion class=\"px-24 lg:w-4\/5 mx-auto\">\n              <app-accordion-item class=\"accordion-group block leading-1 opt-1\" content-class=\"accordion-group__panel wysiwyg-content\">\n          <template #accordion-trigger=\"{ href, visible, setRef, clickHandler }\">\n            <a :href=\"'#' + href\" \n              :ref=\"setRef\"\n              :class=\"['accordion-group__button scroll-mt-160', {'accordion__trigger_active': visible}]\"\n              @click.stop.prevent=\"clickHandler\"\n            >\n            <span class=\"block py-10\">\n              Research Areas\n            <\/span>\n            <span class=\"accordion-group-icon relative flex w-56 h-56 ml-20 justify-center items-center bg-black rounded-full flex-shrink-0\">\n                            <span class=\"w-16 icon-chevron-down\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 406.6c12.5 12.5 32.8 12.5 45.3 0l192-192c12.5-12.5 12.5-32.8 0-45.3s-32.8-12.5-45.3 0L256 338.7 86.6 169.4c-12.5-12.5-32.8-12.5-45.3 0s-12.5 32.8 0 45.3l192 192z\"\/><\/svg>\n              <\/span>\n              <span class=\"w-16 icon-chevron-up\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 105.4c12.5-12.5 32.8-12.5 45.3 0l192 192c12.5 12.5 12.5 32.8 0 45.3s-32.8 12.5-45.3 0L256 173.3 86.6 342.6c-12.5 12.5-32.8 12.5-45.3 0s-12.5-32.8 0-45.3l192-192z\"\/><\/svg>\n              <\/span>\n                          <\/span>\n            <\/a>\n          <\/template>\n          <template #accordion-content>\n            <h2 style=\"text-align: center;\"><strong>Bacterial signal transduction and its role in important physiological and developmental processes<\/strong><\/h2>\n<p><img decoding=\"async\" class=\"size-full wp-image-4831 aligncenter\" title=\"My Document Name\" src=\"\/media\/osteopathic\/szurmant2.jpg\" alt=\"\" width=\"568\" height=\"291\" \/><\/p>\n<p>The Szurmant laboratory has a long-standing interest in bacterial signal transduction. The focus is on the so-called two-component system, a mechanism that is widely utilized by bacteria in many developmental decisions, such as sporulation, genetic competence development, chemotaxis. From a medical perspective, those systems that contribute to antimicrobial resistance, virulence factor expression and those essential for viability are of particular interest.\u00a0 The laboratory has a special focus on the study of WalRK, the only essential two-component signal transduction system of bacteria in the phylum Firmicutes. This phylum includes significant human pathogens such as <em>Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecalis<\/em> and bioterrorism thread <em>Bacillus anthracis<\/em>. Using molecular genetics techniques, we have identified the role this system plays in bacterial physiology. Some efforts in the lab are directed to WalRK\u2019s involvement in antimicrobial resistance of clinical isolates and testing its utility as an antimicrobial drug target.<\/p>\n<hr \/>\n<h2 style=\"text-align: center;\"><strong>Molecular evolution and protein-protein interactions<\/strong><\/h2>\n<p><img decoding=\"async\" class=\"size-full wp-image-4832 aligncenter\" title=\"My Document Name\" src=\"\/media\/osteopathic\/szurmant3.jpg\" alt=\"\" width=\"558\" height=\"280\" \/><\/p>\n<p>Proteins in all organisms undergo concerted interactions in order to execute catalytic, structural, transport and many other essential functions. Numerous genetic diseases are caused by mutations that interfere with wild type protein-protein interactions (PPI). Knowledge on all viral-host cell PPIs, such as those important for COVID-19 infection and disease, would greatly speed up new drug discovery. In bacteria, knowledge on all essential PPIs would greatly enhance the complement of antimicrobial drug targets, in the light of multi-drug resistant bacteria. \u00a0We developed a widely applicable technology termed<em> Direct Coupling Analysis <\/em>(DCA), which in combination with experimental techniques has found wide application in the identification of PPI partners and in solving structures of proteins, protein complexes and alternative conformations of signaling proteins. <em>Direct Coupling Analysis <\/em>relies on the exponentially growing protein sequence databases to mathematically extract contact residue information within and between proteins. We are currently moving this technology forward by trying to understand the amino acid sequence code that dictates not only the structure of PPIs, but also their strength. To this end, we are experimentally sampling directed mutant libraries of thousands of variants of essential interacting protein pairs. In vivo evolution of libraries will inform about the epistatic coupling of amino acid residues on the contact surface between these proteins. Paired with DCA-based computational efforts this approach will provide a leap in our understanding of the protein-sequence code that dictates the strength of an interaction to one day predict all elements of protein function, relying solely on protein sequence.\u00a0 These efforts hold the long-term promise to help identify genetic determinants of complex diseases based on rare variants. In the context of antibiotic resistance, it can be helpful for predicting patterns of adaptive mutations of pathogens (bacteria and viruses) and contribute to the discovery of new therapeutic strategies.<\/p>\n\n          <\/template>\n        <\/app-accordion-item>\n              <app-accordion-item class=\"accordion-group block leading-1 opt-1\" content-class=\"accordion-group__panel wysiwyg-content\">\n          <template #accordion-trigger=\"{ href, visible, setRef, clickHandler }\">\n            <a :href=\"'#' + href\" \n              :ref=\"setRef\"\n              :class=\"['accordion-group__button scroll-mt-160', {'accordion__trigger_active': visible}]\"\n              @click.stop.prevent=\"clickHandler\"\n            >\n            <span class=\"block py-10\">\n              Publications\n            <\/span>\n            <span class=\"accordion-group-icon relative flex w-56 h-56 ml-20 justify-center items-center bg-black rounded-full flex-shrink-0\">\n                            <span class=\"w-16 icon-chevron-down\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 406.6c12.5 12.5 32.8 12.5 45.3 0l192-192c12.5-12.5 12.5-32.8 0-45.3s-32.8-12.5-45.3 0L256 338.7 86.6 169.4c-12.5-12.5-32.8-12.5-45.3 0s-12.5 32.8 0 45.3l192 192z\"\/><\/svg>\n              <\/span>\n              <span class=\"w-16 icon-chevron-up\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 105.4c12.5-12.5 32.8-12.5 45.3 0l192 192c12.5 12.5 12.5 32.8 0 45.3s-32.8 12.5-45.3 0L256 173.3 86.6 342.6c-12.5 12.5-32.8 12.5-45.3 0s-12.5-32.8 0-45.3l192-192z\"\/><\/svg>\n              <\/span>\n                          <\/span>\n            <\/a>\n          <\/template>\n          <template #accordion-content>\n            <p><a href=\"https:\/\/scholar.google.com\/citations?user=MWOh8ZgAAAAJ&amp;hl=en\">Google Scholar link<\/a><\/p>\n<p>Kim, I.M. and <strong>Szurmant, H.<\/strong> A Bacterial Goldilocks Mechanism. <em>Elife<\/em>. 24: e54244, 2020.<\/p>\n<p>Rosales-Hurtado, M., Meffre, P., <strong>Szurmant, H.<\/strong>, Benfodda, Z. Synthesis of Histidine Kinase Inhibitors and Their Biological Properties. <em>Med. Res. Rev.<\/em> 40: 1440-1495, 2020.<\/p>\n<p>Croce, G., Gueudr\u00e9, T., Ruiz Cuevas, M.V., Keidel, V., Figliuzzi, M., <strong>Szurmant, H.<\/strong> and Weigt, M. A Multi-Scale Coevolutionary Approach to Predict Interactions between Protein Domains. <em>PLoS Comput. Biol<\/em>. 15: e1006891, 2019.<\/p>\n<p><strong>Szurmant, H.<\/strong> Evolutionary Couplings of Amino Acid Residues Reveal Structure and Function of Bacterial Signaling Proteins. <em>Mol. Microbiol.<\/em> 112: 432-437, 2019.<\/p>\n<p><strong>Szurmant, H.<\/strong> and Weigt, M. Inter-Residue, Inter-Protein and Inter-Family Coevolution: Bridging the Scales. <em>Curr. Opin. Struct. Biol.<\/em> 2018:26-32, 2018.<\/p>\n<p>Uguzzoni, G., John Lovis, S., Oteri, F., Schug, A.*, <strong>Szurmant, H.<\/strong>* and Weigt, M.* Large-Scale Identification of Coevolution Signals across Homo-Oligomeric Protein Interfaces by Direct Coupling Analysis. <em>Proc. Natl. Acad. Sci. USA<\/em>. 114: E2662-E2671, 2017. *Co-corresponding authors.<\/p>\n<p>Boibessot, T., Zschiedrich, C.P., Lebeau, A., B\u00e9nim\u00e8lis, D., Dunyach-R\u00e9my, C., Lavigne, J.P., <strong>Szurmant, H.<\/strong>*, Benfodda, Z.* and Meffre, P.*. The Rational Design, Synthesis, and Antimicrobial Properties of Thiophene Derivatives That Inhibit Bacterial Histidine Kinases. <em>J. Med. Chem. <\/em>59: 8830-8847, 2016. *Co-corresponding authors<\/p>\n<p>Zschiedrich, C. P., Keidel, V. and <strong>Szurmant, H.<\/strong> Molecular Mechanisms of Two-component Signal Transduction. <em>J. Mol. Biol.<\/em> 428: 3752-75, 2016.<\/p>\n<p>Feinauer, C., <strong>Szurmant, H.<\/strong>, Weigt, M. and Pagnani, A. Inter-Protein Sequence Co-Evolution Predicts Known Physical Interactions in Bacterial Ribosomes and the Trp Operon <em>PLoS ONE. <\/em>11<strong>: <\/strong>e0149166, 2016.<\/p>\n<p>Pokkuluri P.R., Dwulit-Smith, J., Duke, N.E., Wilton, R., Mack, J.C., Bearden, J., Rakowski, E., Babnigg, G., <strong>Szurmant, H.<\/strong>, Joachimiak, A., and Schiffer, M. Analysis of Periplasmic Sensor Domains from <em>Anaeromyxobacter dehalogenans <\/em>2CP-C: Structure of One Sensor Domain from a Histidine Kinase and Another from a Chemotaxis Protein. <em>Microbiology Open<\/em>. 2<strong>: <\/strong>766-77, 2013.<\/p>\n<p>Wu, R., Gu, M., Wilton, R., Babnigg, G., Kim, Y., Pokkuluri, P.R., <strong>Szurmant, H.<\/strong>, Joachimiak, A., Schiffer, M. Insight into the Sporulation Phosphorelay: Crystal Structure of the Sensor Domain of <em>Bacillus subtilis <\/em>Histidine Kinase KinD. <em>Protein Sci. <\/em>22: 564-76, 2013.<\/p>\n<p><strong>Szurmant, H.<\/strong> and Hoch, J.A. Statistical Analyses of Protein Databases Identify Structures and Mechanisms in Signal Activation of Sensor Histidine Kinases. <em>Mol. Microbiol. <\/em>87: 707-12, 2013.<\/p>\n<p>Dago, A.E., Schug, A., Procaccini, A., Hoch, J.A., Weigt, M., and <strong>Szurmant, H.<\/strong> The Structural Basis of Histidine Kinase Autophosphorylation: Integrating Genomics, Molecular Dynamics and Mutagenesis. <em>Proc. Natl. Acad. Sci USA <\/em>109: E1733-42, 2012.<\/p>\n<p>Diaz, A.R., Core, L., Jiang, M., Morelli, M., Chiang, C.H., <strong>Szurmant, H.<\/strong>, and Perego, M. <em>Bacillus subtilis <\/em>RapA Phosphatase Domain Interaction with its Substrate, Phosphorylate Spo0F, and its Inhibitor, the PhrA peptide. <em>J. Bacteriol. <\/em>194: 1378-88, 2012.<\/p>\n<p>Fukushima, T., Furihata, I., Emmins, R., Daniel, R.A., Hoch, J.A., and <strong>Szurmant, H.<\/strong> A Role for the Essential YycG Sensor Histidine Kinase In Sensing Cell Division. <em>Mol.<\/em> <em>Microbiol. <\/em>79: 503-22, 2011.<\/p>\n<p>Procaccini, A., Lunt, B., <strong>Szurmant, H.<\/strong>, Hwa, T., and Weigt, M. Dissecting the Specificity of Protein-Protein Interaction in Bacterial Two-Component Signaling: Orphans and Crosstalks. <em>PLoS One <\/em>6: e19729, 2011.<\/p>\n<p>Wilson, A.C. and <strong>Szurmant, H.<\/strong> Transposon Systems for Random Mutagenesis of <em>Bacillus subtilis<\/em>. <em>Methods Mol. Biol. <\/em>765: 359-71, 2011.<\/p>\n<p><strong>Szurmant, H.<\/strong> and Hoch, J.A. Interaction Fidelity in Two-Component Signaling. <em>Curr. Opin. Microbiol. <\/em>13: 190-7, 2010.<\/p>\n<p>Schug, A., Weigt, M., Hoch, J.A., Onuchic, J.N., Hwa, T., and <strong>Szurmant, H.<\/strong> Computational Modeling of Phosphotransfer Complexes in Two-Component Signaling. <em>Methods Enzymol. <\/em>471: 43-58, 2010.<\/p>\n<p>Lunt, B., <strong>Szurmant, H.<\/strong>, Procaccini, A., Hoch, J.A., Hwa, T., Weigt, M. Inference of Direct Residue Contacts in Two-Component Signaling. <em>Methods Enzymol. <\/em>471: 17-41, 2010.<\/p>\n<p>Chang, C., Tesar, C., Gu, M., Babnig, G., Joachimiak, A., Pokkuluri, P.R., <strong>Szurmant, H.<\/strong>, and Schiffer, M. Periplasmic PAS Domains: A Dominant Structural Family Governing Signal Transduction. <em>J. Bacteriol. <\/em>192: 1156-9, 2010.<\/p>\n<p>Schug, A., Weigt, M., Onuchic, J.N., Hwa, T., and <strong>Szurmant, H.<\/strong> High Resolution Protein Complexes From Integrating Genomic Information With Molecular Simulation. <em>Proc.<\/em> <em>Natl. Acad. Sci. USA <\/em>106: 22124-9, 2009.<\/p>\n<p>Weigt, M., White, R. A., <strong>Szurmant, H.<\/strong>, Hoch, J.A., and Hwa, T. Identification of Direct Residue Contacts in Protein-Protein Interaction By Message Passing. <em>Proc. Natl. Acad. Sci. USA <\/em>106: 67-72, 2009.<\/p>\n<p><strong>Szurmant, H.<\/strong>, Bobay, B.G., White, R. A., Sullivan, D.M., Thompson, R.J., Hwa, T., Hoch, J.A., and Cavanagh, J. Co-evolving Motions at Protein-Protein Interfaces of Two- Component Signaling Systems Identified by Covariance Analysis. <em>Biochemistry<\/em>, 47: 7782-4, 2008. Selected as \u201chot article\u201d by the editorial board.<\/p>\n<p>Fukushima, T., <strong>Szurmant, H.<\/strong>, Kim, E.J., Perego, M., and Hoch, J.A. A Sensor Histidine Kinase Coordinates Cell Wall Architecture with Cell Division in <em>Bacillus subtilis<\/em>. <em>Mol.<\/em> <em>Microbiol. <\/em>69: 621-32 (Including Cover Image), 2008.<\/p>\n<p><strong>Szurmant, H.<\/strong>, Bu, L., Brooks, C.L., and Hoch, J.A. An Essential Sensor Histidine Kinase Controlled By Transmembrane Helix Interaction with its Auxiliary Proteins. <em>Proc. Natl.<\/em> <em>Acad. Sci. USA <\/em>105: 5891-6, 2008.<\/p>\n<p><strong>Szurmant, H.<\/strong>, White, R.A., and Hoch, J.A. Sensor Complexes Regulating Twocomponent Signal Transduction. <em>Curr. Opin. Struct. Biol. <\/em>17: 706-15, 2007.<\/p>\n<p>Santelli, E., Liddington, R.C., Mohan, M.A., Hoch, J.A., and <strong>Szurmant, H.<\/strong> The Crystal Structure of <em>Bacillus subtilis <\/em>YycI Reveals a Common Fold for Two Members of an Unusual Class of Histidine Kinase Regulatory Proteins. <em>J. Bacteriol. <\/em>289: 3280-9, 2007.<\/p>\n<p><strong>Szurmant, H.<\/strong>, Mohan, M.A., Imus, P.M., and Hoch, J.A. YycH and YycI Interact to Regulate the Essential YycFG Two-Component System. <em>J. Bacteriol. <\/em>289: 3290-5, 2007.<\/p>\n<p>White, R. A., <strong>Szurmant, H.<\/strong>, Hoch, J.A., and Hwa, T. Features of Protein-protein Interactions in Two-Component Signaling Deduced from Genomic Libraries. <em>Methods<\/em> <em>Enzymol. <\/em>422: 75-101, 2007.<\/p>\n<p><strong>Szurmant, H.<\/strong>, Fukushima, T., M.A., and Hoch, J.A. The Essential YycFG Two- Component System in <em>Bacillus subtilis<\/em>. <em>Methods Enzymol. <\/em>422: 396-417, 2007.<\/p>\n<p><strong>Szurmant, H.<\/strong>, Zhao, H., Mohan, M.A., Hoch, J.A., and Varughese, K.I. The Crystal Structure of YycH Involved in the Regulation of the Essential YycFG Two-Component System in <em>Bacillus subtilis <\/em>reveals a Novel Tertiary Structure. <em>Protein Sci. <\/em>15: 929-34 (Including Cover Image), 2006.<\/p>\n<p>W\u00f6rner, K., <strong>Szurmant, H.<\/strong>, Chiang, C., and Hoch, J.A. Phosphorylation and Functional Analysis of the Sporulation Initiation Factor Spo0A from <em>Clostridium botulinum<\/em>. <em>Mol.<\/em> <em>Microbiol.<\/em>, 59: 1000-12, 2006.<\/p>\n<p><strong>Szurmant, H.<\/strong>, Nelson, K., Kim, E.J., Perego, M., and Hoch, J.A. YycH Regulates the Activity of the Essential YycFG Two-Component System in <em>Bacillus subtilis. J. Bacteriol<\/em>. 187: 5419-26, 2005.<\/p>\n<p><strong>Szurmant, H.<\/strong> and Ordal, G.W. The Diversity in Chemotaxis Mechanisms among the Bacteria and Archaea. <em>Microbiol. Mol. Biol. Rev. <\/em>68: 301-19, 2004.<\/p>\n<p><strong>Szurmant, H.<\/strong>, Muff, T., and Ordal, G.W. <em>Bacillus subtilis <\/em>CheC and FliY are Members of a Novel Class of CheY-P Hydrolyzing Proteins in the Chemotactic Signal Transduction Cascade. <em>J. Biol. Chem. <\/em>279: 21787-92, 2004.<\/p>\n<p><strong>Szurmant, H.<\/strong>, Bunn, M.W., Cho, S.H., and Ordal, G.W. Ligand Induced Conformational Changes in the <em>Bacillus subtilis <\/em>Chemotaxis Receptor McpB Determined by Disulfide Crosslinking in vivo. <em>J. Mol. Biol. <\/em>344: 919-28, 2004.<\/p>\n<p><strong>Szurmant, H.<\/strong>, Bunn, M.W., Cannistraro, V.J., and Ordal, G.W. <em>Bacillus subtilis<\/em> Hydrolyzes CheY-P at the Place of its Action, the Flagellar Switch. <em>J. Biol. Chem. <\/em>278: 48611-6, 2003.<\/p>\n<p>Zimmer, M.A., <strong>Szurmant, H.<\/strong>, Saulmon, M.M., Collins, M. A., Bant, J.S., and Ordal, G.W. The Role of Heterologous Receptors in McpB-Mediated Signaling in <em>Bacillus subtilis <\/em>Chemotaxis. <em>Mol. Microbiol. <\/em>45: 555-69, 2002.<\/p>\n\n          <\/template>\n        <\/app-accordion-item>\n          <\/app-accordion>\n  <\/div>\n<\/section>\n\n<section class=\"faculty-card my-48 md:my-72\">\n  <div class=\"container\">\n        <div class=\"md:flex md:justify-between md:items-end mb-20\">\n      <h2 class=\"t-h2-sans  text-red md:mr-40\">HENDRIK SZURMANT, PhD<\/h2>\n    <\/div>\n            <div class=\"md:flex md:flex-wrap px-12\">\n                                                      \n                                                        \n      <div class=\"px-12 mt-24 w-full md:w-1\/2\">\n        <div class=\"sm:flex w-full py-32 border-b-1 border-gray\">\n                                            <a href=\"\/bios\/?bio=hszurmant\" class=\"card block w-256 sm:w-1\/3 pl-12 md:pl-0 pr-12 md:pr-18 mr-auto md:mr-0\">\n                <div class=\"rounded-5 md:rounded-8 overflow-hidden\">\n                    <img decoding=\"async\" class=\"card__image w-full h-256 sm:h-192 md:h-144 lg:h-192 xl:h-256 object-cover\" alt=\"Portrait of HENDRIK SZURMANT, PhD\" src=\"\/media\/westernu\/images\/staff\/hszurmant.jpg\" \/>\n                <\/div>\n            <\/a>\n                                <div class=\"w-full h-full md:w-2\/3 pl-12 md:pl-18 mt-18 sm:mt-0\">\n            <p class=\"t-h2-serif leading-120\">\n                            <a href=\"\/bios\/?bio=hszurmant\" class=\"text-red-light hover:text-red\">HENDRIK SZURMANT, PhD<\/a>\n                          <\/p>\n            <div class=\"mt-16 t-p2\">\n                            <p class=\"font-bold\">College of Osteopathic Medicine of the Pacific<\/p>\n                                          <p class=\"font-bold\">Associate Professor of Microbiology<\/p>\n                                          <p>\n                <a href=\"tel:909-706-3938\" class=\"hover:underline\">909-706-3938<\/a>\n              <\/p>\n                                                        <p>\n                <a href=\"mailto:hszurmant@westernu.edu\" class=\"hover:underline\">hszurmant@westernu.edu <\/a>\n              <\/p>\n                                        <\/div>\n          <\/div>\n        <\/div>\n      <\/div>\n          <\/div>\n      <\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":34,"featured_media":0,"parent":8215,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-4825","page","type-page","status-publish","hentry"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages\/4825","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/users\/34"}],"replies":[{"embeddable":true,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/comments?post=4825"}],"version-history":[{"count":13,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages\/4825\/revisions"}],"predecessor-version":[{"id":14081,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages\/4825\/revisions\/14081"}],"up":[{"embeddable":true,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages\/8215"}],"wp:attachment":[{"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/media?parent=4825"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}