{"id":4321,"date":"2020-03-25T10:17:10","date_gmt":"2020-03-25T17:17:10","guid":{"rendered":"https:\/\/stagewp.westernu.edu\/osteopathic\/?page_id=4321"},"modified":"2024-04-16T10:33:26","modified_gmt":"2024-04-16T17:33:26","slug":"wagner","status":"publish","type":"page","link":"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/wagner\/","title":{"rendered":"Wagner Lab"},"content":{"rendered":"<section class=\"hero-secondary\">\n  <div class=\"relative bg-black\">\n                <img decoding=\"async\" class=\"adhere object-cover\" src=\"\/media\/osteopathic\/wagner-lab.png\" srcset=\"\" sizes=\"100vw\" alt=\"wagner-lab image\">\n      <div class=\"adhere gradient-overlay\"><\/div>\n        <div class=\"relative container pt-56 md:pt-112 pb-144 md:pb-192\">\n      <div class=\"md:flex md:justify-between px-12\">\n        <div class=\"sm:w-8\/12 md:w-6\/12 xl:w-5\/12 px-12\">\n                      <div class=\"mb-20\">\n              <div class=\"block font-sans font-bold text-14 text-white leading-130\">\n  <span class=\"inline-block\">\n    <a href=\"\/\" class=\"block hover:underline\">WesternU<\/a>\n  <\/span>\n      <span class=\"inline-block mx-6\">\/<\/span>\n          <a href=\"https:\/\/stagewp.westernu.edu\/osteopathic\" class=\"inline-block hover:underline\">College of Osteopathic Medicine of the Pacific (COMP) and COMP-Northwest<\/a>\n          <span class=\"inline-block mx-6\">\/<\/span>\n      <a href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/\" class=\"inline-block hover:underline\">Research<\/a>\n    <span class=\"inline-block mx-6\">\/<\/span>\n      <a href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/\" class=\"inline-block hover:underline\">Research Labs<\/a>\n    <span class=\"inline-block mx-6\">\/<\/span>\n    <span class=\"inline-block\">Wagner Lab<\/span>\n  <\/div>            <\/div>\n                                        <h1 class=\"t-h1-sans  text-white\">Wagner Lab<\/h1>\n        <\/div>\n              <\/div>\n    <\/div>\n  <\/div>\n<\/section>\n\n\n<section class=\"relative my-48 md:my-72\">\n  <div class=\"container\">\n    <div class=\"lg:flex px-12 lg:justify-start\">\n              <div class=\"w-full lg:w-5\/12 2xl:w-4\/12 -mt-80 md:-mt-112 mb-48 px-12 lg:pr-32\">\n                              <nav class=\"py-28 px-40 2xl:px-64 rounded-5 md:rounded-8 bg-gray-darker text-white mb-40\">\n            <ul>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/\" target=\"_self\" rel=\"noopener\">Research Labs<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/bi-lab\/\" target=\"_self\" rel=\"noopener\">Bi Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/elsalanty-lab\/\" target=\"_self\" rel=\"noopener\">Elsalanty Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/fricano-lab\/\" target=\"_self\" rel=\"noopener\">Fricano Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/nalley-lab\/\" target=\"_self\" rel=\"noopener\">Nalley Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/peterfy-lab\/\" target=\"_self\" rel=\"noopener\">Peterfy Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/scott-lab\/\" target=\"_self\" rel=\"noopener\">Scott Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/sperling-lab\/\" target=\"_self\" rel=\"noopener\">Sperling Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/steinauer-lab\/\" target=\"_self\" rel=\"noopener\">Steinauer Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/szurmant-lab\/\" target=\"_self\" rel=\"noopener\">Szurmant Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/venketaraman-lab\/\" target=\"_self\" rel=\"noopener\">Venketaraman Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/wagner\/\" target=\"_self\" rel=\"noopener\">Wagner Lab<\/a>\n                <\/li>\n                                              <li class=\"block my-8\">\n                  <a class=\"text-22 font-normal leading-130 tracking-20 text-white hover:text-yellow transition-default\" href=\"https:\/\/stagewp.westernu.edu\/osteopathic\/research\/labs\/wedel-lab\/\" target=\"_self\" rel=\"noopener\">Wedel Lab<\/a>\n                <\/li>\n                          <\/ul>\n          <\/nav>\n                            <\/div>\n      \n      \n                  \n                  \n      <div class=\"w-full px-24 wysiwyg-content lg:w-7\/12 2xl:w-7\/12\">\n        <p>The Wagner Laboratory focuses on the synaptic and hormonal determinants underlying the central control of energy homeostasis.\u00a0 The research conducted in his lab probes the function of nutrient- and palatability-sensing circuits in the brain, and how dysregulation of these circuits helps establish a link between diet-induced obesity and food addiction.<\/p>\n\n      <\/div>\n    <\/div>\n  <\/div>\n<\/section>\n\n\n\n\n\n\n\n<section class=\"relative my-48 md:my-72\" vue-app>\n  <div class=\"container\">\n        <app-accordion class=\"px-24 lg:w-4\/5 mx-auto\">\n              <app-accordion-item class=\"accordion-group block leading-1 opt-1\" content-class=\"accordion-group__panel wysiwyg-content\">\n          <template #accordion-trigger=\"{ href, visible, setRef, clickHandler }\">\n            <a :href=\"'#' + href\" \n              :ref=\"setRef\"\n              :class=\"['accordion-group__button scroll-mt-160', {'accordion__trigger_active': visible}]\"\n              @click.stop.prevent=\"clickHandler\"\n            >\n            <span class=\"block py-10\">\n              Research Areas\n            <\/span>\n            <span class=\"accordion-group-icon relative flex w-56 h-56 ml-20 justify-center items-center bg-black rounded-full flex-shrink-0\">\n                            <span class=\"w-16 icon-chevron-down\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 406.6c12.5 12.5 32.8 12.5 45.3 0l192-192c12.5-12.5 12.5-32.8 0-45.3s-32.8-12.5-45.3 0L256 338.7 86.6 169.4c-12.5-12.5-32.8-12.5-45.3 0s-12.5 32.8 0 45.3l192 192z\"\/><\/svg>\n              <\/span>\n              <span class=\"w-16 icon-chevron-up\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 105.4c12.5-12.5 32.8-12.5 45.3 0l192 192c12.5 12.5 12.5 32.8 0 45.3s-32.8 12.5-45.3 0L256 173.3 86.6 342.6c-12.5 12.5-32.8 12.5-45.3 0s-12.5-32.8 0-45.3l192-192z\"\/><\/svg>\n              <\/span>\n                          <\/span>\n            <\/a>\n          <\/template>\n          <template #accordion-content>\n            <p>The Wagner lab focuses on the central regulation of energy balance (i.e., energy intake and expenditure). We probe the influence of sex and the activational effects of gonadal hormones on the signaling of critical neuropeptides (e.g., nociceptin\/orphanin FQ (N\/OFQ), pituitary adenylyl cyclase-activating polypeptide (PACAP)) and neuromodulators (e.g., endocannabinoids) within the homeostatic (i.e., nutrient sensing) and hedonic (i.e., palatability sensing) energy balance circuits. We also evaluate the impact of energy status across the spectrum \u2013 from negative energy balance (e.g., fasting) to positive energy balance (e.g., diet-induced obesity) \u2013 on the functioning of these distinct yet overlapping circuits. We use state-of-the-art optogenetic and chemogenetic techniques in strategically determined transgenic animal models to elicit cell type-specific excitation or inhibition of critical neuroanatomical substrates within these circuits and ascertain the corresponding changes in energy intake, meal pattern and energy expenditure. Our work provides direct translational insights into the underpinnings to clinically relevant conditions such as HIV\/AIDS-related cachexia and diabetes\/insulin resistance.<\/p>\n<p>&nbsp;<\/p>\n<hr \/>\n<h3 style=\"text-align: center;\"><strong>Nociceptin\/Orphanin FQ and Energy Balance<\/strong><\/h3>\n<article class=\"_setcol2\">\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" class=\"alignright wp-image-4373\" style=\"font: inherit; margin: 0.5em 1em 0.5em 0px; padding: 0px; border: 0px; height: 197px; text-align: left; color: #1e1e1e; text-transform: none; text-indent: 0px; letter-spacing: normal; text-decoration: none; vertical-align: baseline; max-width: 100%; float: left;\" title=\"My Document Name\" src=\"\/media\/osteopathic\/Wagner_Lab1.jpg\" alt=\"\" width=\"400\" height=\"251\" \/><\/article>\n<article class=\"_setcol2\">\n<p>We have shown over the years that N\/OFQ profoundly impacts neuronal activity within critical anorexigenic (i.e., appetite suppressing) circuits involved in the hypothalamic control of energy homeostasis. This occurs through both presynaptic and postsynaptic mechanisms. As shown below, optogenetic stimulation of ARC N\/OFQ neurons inhibits anorexigenic proopiomelanocortin (POMC) neurons in double transgenic N\/OFQ-cre\/ eGFP POMC mice. The photomicrograph below illustrates the N\/OFQ immunostaining in the ARC visualized with AF546 and colocalized with channelrhodopsin (ChR2; visualized with an eYFP reporter signal. The photostimulation elicits a robust and reversible, nociceptin opioid (NOP) receptor-mediated outward current in the POMC neurons that reverses polarity near the equilibrium potential for K+ and is accompanied by increased slope conductance.\u00a0<\/p>\n<\/article>\n<p>&nbsp;<\/p>\n<article class=\"_setcol2\">\n<p>Within the hedonic circuitry, our recent findings demonstrate that N\/OFQ also inhibits mesolimbicortical (A10) dopamine neurons in the ventral tegmental area (VTA) that influence natural and drug-induced reward as well as addiction. This occurs upon activation of NOP receptors via the same mechanism as describe above for POMC neurons.<\/p>\n<\/article>\n<article class=\"_setcol2\"><img decoding=\"async\" class=\"alignleft wp-image-4371\" style=\"font: inherit; margin: 0.5em 0px 0.5em 1em; padding: 0px; border: 0px; height: 146px; text-align: left; color: #1e1e1e; text-transform: none; text-indent: 0px; letter-spacing: normal; text-decoration: none; vertical-align: baseline; max-width: 100%; float: right;\" title=\"My Document Name\" src=\"\/media\/osteopathic\/Wagner_Lab2.jpg\" alt=\"\" width=\"400\" height=\"187\" \/><\/article>\n<p>&nbsp;<\/p>\n<hr \/>\n<h3 style=\"text-align: center;\"><strong>Feeding Circuitry<\/strong><\/h3>\n<article class=\"_setcol2\">\n<p>As mentioned above, one of our main research objectives is focused on the critical anorexigenic circuit within the hypothalamic energy balance circuitry comprising upstream PACAP neurons in the ventromedial nucleus that synapse with POMC neurons in the arcuate nucleus. Chemogenetic activation of PACAP neurons increases cFOS expression and the firing rate within these cells. This results in decreased energy intake and meal size, which is accompanied by an increase in energy expenditure.<\/p>\n<\/article>\n<article class=\"_setcol2\">\n<p><img decoding=\"async\" class=\"aligncenter wp-image-4372\" title=\"My Document Name\" src=\"\/media\/osteopathic\/Wagner_Lab3.jpg\" alt=\"\" width=\"400\" height=\"299\" \/><\/p>\n<\/article>\n<hr \/>\n<article class=\"_setcol2\"><img decoding=\"async\" class=\"aligncenter size-full wp-image-4442\" title=\"My Document Name\" src=\"\/media\/osteopathic\/chart1.jpg\" alt=\"\" width=\"701\" height=\"457\" \/><\/article>\n<article class=\"_setcol2\">While the homeostatic energy balance circuitry within the hypothalamus is principally concerned with need, the hedonic energy balance circuitry, on the other hand, is focuses more on the want. A10 dopamine neurons have been implicated in food addiction and impulsivity. As shown below, intermittent short-term exposure to a \u201cWesternized\u201d high-fat diet elicits a dramatic escalation in consumption, such that subjects end up eating considerably more of their daily intake during just that one exposure. N\/OFQ administered into the VTA reduces this binge-like escalation via a NOP receptor-mediated mechanism in a manner dependent on sex, the gonadal hormone milieu and dietary status.<\/article>\n<hr \/>\n<h3 style=\"text-align: center;\"><strong>What We Want to Test<\/strong><\/h3>\n<p>Our current studies regarding N\/OFQ are aimed to test the hypothesis that N\/OFQ regulates homeostatic energy balance circuitry in a sex- and diet-dependent manner by specifically determining whether:<\/p>\n<ul>\n<li>chemostimulation of ARC N\/OFQ neurons to stimulate energy intake and reduce energy expenditure in chow- or \u201cWesternized\u201d, high-fat diet-fed male and female animals,<\/li>\n<li>photostimulation of ARC N\/OFQ neurons to directly hyperpolarize in POMC neurons via activation of GIRK channels<\/li>\n<\/ul>\n<p>We also are looking to test the hypothesis that N\/OFQ modulates circuits regulating energy balance and related appetitive behavioral processes in a sex- and diet-dependent manner as assessed by the ability of:<\/p>\n<ul>\n<li>chemostimulation of VTA N\/OFQ neurons to blunt binge-eating behavior caused by intermittent, short-term exposure to HFD,<\/li>\n<li>exogenously administered N\/OFQ to directly inhibit A10 dopamine neurons via activation of GIRK channels.<\/li>\n<\/ul>\n\n          <\/template>\n        <\/app-accordion-item>\n              <app-accordion-item class=\"accordion-group block leading-1 opt-1\" content-class=\"accordion-group__panel wysiwyg-content\">\n          <template #accordion-trigger=\"{ href, visible, setRef, clickHandler }\">\n            <a :href=\"'#' + href\" \n              :ref=\"setRef\"\n              :class=\"['accordion-group__button scroll-mt-160', {'accordion__trigger_active': visible}]\"\n              @click.stop.prevent=\"clickHandler\"\n            >\n            <span class=\"block py-10\">\n              Publications\n            <\/span>\n            <span class=\"accordion-group-icon relative flex w-56 h-56 ml-20 justify-center items-center bg-black rounded-full flex-shrink-0\">\n                            <span class=\"w-16 icon-chevron-down\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 406.6c12.5 12.5 32.8 12.5 45.3 0l192-192c12.5-12.5 12.5-32.8 0-45.3s-32.8-12.5-45.3 0L256 338.7 86.6 169.4c-12.5-12.5-32.8-12.5-45.3 0s-12.5 32.8 0 45.3l192 192z\"\/><\/svg>\n              <\/span>\n              <span class=\"w-16 icon-chevron-up\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 105.4c12.5-12.5 32.8-12.5 45.3 0l192 192c12.5 12.5 12.5 32.8 0 45.3s-32.8 12.5-45.3 0L256 173.3 86.6 342.6c-12.5 12.5-32.8 12.5-45.3 0s-12.5-32.8 0-45.3l192-192z\"\/><\/svg>\n              <\/span>\n                          <\/span>\n            <\/a>\n          <\/template>\n          <template #accordion-content>\n            <p>\u2022 Chang R, Hernandez J, Gastelum C, Guadagno K, Perez L, Wagner E, J: Pituitary Adenylate Cyclase-Activating Polypeptide Excites Proopiomelanocortin Neurons: Implications for the Regulation of Energy Homeostasis. Neuroendocrinology 2020. doi: 10.1159\/000506367<\/p>\n<p>\u2022 Hernandez J, Fabelo C, Perez L, Moore C, Chang R, Wagner EJ. Nociceptin\/orphanin FQ modulates energy homeostasis through inhibition of neurotransmission at VMN SF-1\/ARC POMC synapses in a sex- and diet-dependent manner. Biol Sex Differ. 2019;10(1):9. Published 2019 Feb 12. doi:10.1186\/s13293-019-0220-3<\/p>\n<p>\u2022 Fabelo C, Hernandez J, Chang R, et al. Endocannabinoid Signaling at Hypothalamic Steroidogenic Factor-1\/Proopiomelanocortin Synapses Is Sex- and Diet-Sensitive. Front Mol Neurosci. 2018;11:214. Published 2018 Jun 19. doi:10.3389\/fnmol.2018.00214<\/p>\n<p>\u2022 Qiu J. Bosch MA, Meza C, Navarro U, Nestor CC, Wagner EJ, R\u00f8nnekleiv OK and Kelly MJ. Estradiol Protects Proopiomelanocortin Neurons Against Insulin Resistance. Endocrinology. February 2018, 159(2):647-664.<\/p>\n<p>\u2022 Qiu J, Wagner EJ, R\u00f8nnekleiv OK, Kelly MJ. Insulin and Leptin Excite Anorexigenic Pro-Opiomelanocortin Neurones via Activation of TRPC5 Channels. Journal of Neuroendocrionolgy. June 2017. DOI: 10.1111\/jne.12501.<\/p>\n<p>\u2022 Conde K, Fabelo C, Krause WC, Propst R, Goethel J, Fischer D, Hur J, Meza C, Ingraham HA, Wagner EJ. Testosterone Rapidly Augments Retrograde Endocannabinoid Signaling in Proopiomelanocortin Neurons to Suppress Glutamatergic Input from Steroidogenic Factor-1 Neurons via Upregulation of Diacylglycerol Lipase-a. Neuroendocrinology. 2016 Nov. 21 PMID:27871072<\/p>\n<p>\u2022 Wagner EJ. Sex differences in cannabinoid-regulated biology: A focus on energy homeostasis. Front Neuroendocrinol. 2016 Jan;40:101-9. doi 10.1016\/j.yfrbe.2106.01.003. Epub 2016 Jan 19.<\/p>\n<p>\u2022 Conde K, Meza C, Kelly MJ, Sinchak K, Wagner EJ. Estradiol Rapidly Attenuates ORL-1 Receptor-Mediated Inhibition of Proopiomelanocortin Neurons via Gq-Coupled, Membrane-Initiated Signaling. Neuroendocrinology. 2016 Jan 16. PMID:26765570<\/p>\n<p>\u2022 Mela V, Vargas A, Meza C, Kachani M, Wagner EJ. Modulatory influences of estradiol and other anorexigenic hormones on metabotropic:Gi\/O-coupled receptor function in the hypothalamic control of energy homeostasis. J Steroid Biochem Mol Biol. 2015 Jul 28. pii: S0960-0760(15)30027-3. doi:10.1016\/j.jsbmb.2015.07.014.<\/p>\n<p>\u2022 Borgquist A, Meza C, Wagner EJ. Role of neuronal nitric oxide synthase in the estrogenic attenuation of cannabinoid-induced changes in energy homeostasis. J Neurophysiol. 2015 Feb 1;113(3):904-14. doi: 10.1152\/jn.00615.2014. Epub 2014 Nov 12.<\/p>\n<p>\u2022 Borgquist A, Meza C, Wagner EJ. The role of AMP-activated protein kinase (AMPK) in the androgenic potentiation of cannabinoid-induced changes in energy homeostasis. Am J Physiol Endocrinol Metab. 2014 Dec 30:ajpendo.00421.2014. doi: 10.1152\/ajpendo.00421.2014. https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25550281<\/p>\n<p>\u2022 Qiu J, Zhang C, Borgquist A, Nestor CC, Smith AW, Bosch MA, Ku S, Wagner EJ, R\u00f8nnekleiv OK, Kelly MJ. Insulin excites anorexigenic proopiomelanocortin neurons via activation of canonical transient receptor potential channels. Cell Metab. 2014 Apr 1;19(4):682-93. doi: 10.1016\/j.cmet.2014.03.004.<\/p>\n<p>\u2022 Borgquist A, Kachani M, Tavitian N, Sinchak K, Wagner EJ. Estradiol negatively modulates the pleiotropic actions of orphanin FQ\/nociceptin at proopiomelanocortin synapses. Neuroendocrinology. 2013;98(1):60-72. doi: 10.1159\/000351868. Epub 2013 Jul 2.<\/p>\n<p>\u2022 Sinchak K, Wagner EJ. Estradiol signaling in the regulation of reproduction and energy balance. Front Neuroendocrinol. 2012 Oct;33(4):342-63. doi: 10.1016\/j.yfrne.2012.08.004. Epub 2012 Sep 7.<\/p>\n<p>\u2022 Viveros MP, Mendrek A, Paus T, Lopez-Rodriguez AB, Marco EM, Yehuda R, Cohen H, Lehrner A, Wagner EJ: A comparative, developmental, and clinical perspective of neurobehavioral sexual dimorphisms. Frontiers in Neuroscience 2012 Jun 12;6:84. doi: 10.3389\/fnins.2012.00084.<\/p>\n<p>\u2022 Washburn N, Borgquist A, Wang K, Jeffery GS, Kelly MJ, Wagner EJ: Receptor subtypes and signal transduction mechanisms contributing to the estrogenic attenuation of cannabinoid-induced changes in energy homeostasis. Neuroendocrinology 2013;97(2):160-75. doi: 10.1159\/000338669.<\/p>\n<p>\u2022 Viveros MP, Bermudez-Silva FJ, Lopez-Rodriguez AB and Wagner EJ. The Endocannabinoid System as Pharmacological Target Derived from Its CNS Role in Energy Homeostasis and Reward. Applications in Eating Disorders and Addiction. Pharmaceuticals 2011, 4(8), 1101-1136; doi:10.3390\/ph4081101.<\/p>\n<p>\u2022 Viveros MP, Marco E, Lopez-Gallardo M, Garcia-Segura LM and Wagner EJ. Framework for sex differences in adolescent neurobiology: A focus on cannabinoids. Neuroscience and Biobehavioral Reviews 2011, 35 (8), pp. 1740-1751.<\/p>\n<p>\u2022 Jeffery GS, Peng KC and Wagner EJ. The role of phosphatidylinositol-3-kinase and AMP-activated kinase in the rapid estrogenic attenuation of cannabinoid-induced changes in energy homeostasis. Pharmaceuticals 2011, 4 (4), pp. 630-651.<\/p>\n\n          <\/template>\n        <\/app-accordion-item>\n              <app-accordion-item class=\"accordion-group block leading-1 opt-1\" content-class=\"accordion-group__panel wysiwyg-content\">\n          <template #accordion-trigger=\"{ href, visible, setRef, clickHandler }\">\n            <a :href=\"'#' + href\" \n              :ref=\"setRef\"\n              :class=\"['accordion-group__button scroll-mt-160', {'accordion__trigger_active': visible}]\"\n              @click.stop.prevent=\"clickHandler\"\n            >\n            <span class=\"block py-10\">\n              Lab Members\n            <\/span>\n            <span class=\"accordion-group-icon relative flex w-56 h-56 ml-20 justify-center items-center bg-black rounded-full flex-shrink-0\">\n                            <span class=\"w-16 icon-chevron-down\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 406.6c12.5 12.5 32.8 12.5 45.3 0l192-192c12.5-12.5 12.5-32.8 0-45.3s-32.8-12.5-45.3 0L256 338.7 86.6 169.4c-12.5-12.5-32.8-12.5-45.3 0s-12.5 32.8 0 45.3l192 192z\"\/><\/svg>\n              <\/span>\n              <span class=\"w-16 icon-chevron-up\">\n                <svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 512 512\"><!--!Font Awesome Free 6.6.0 by @fontawesome - https:\/\/fontawesome.com License - https:\/\/fontawesome.com\/license\/free Copyright 2024 Fonticons, Inc.--><path d=\"M233.4 105.4c12.5-12.5 32.8-12.5 45.3 0l192 192c12.5 12.5 12.5 32.8 0 45.3s-32.8 12.5-45.3 0L256 173.3 86.6 342.6c-12.5 12.5-32.8 12.5-45.3 0s-12.5-32.8 0-45.3l192-192z\"\/><\/svg>\n              <\/span>\n                          <\/span>\n            <\/a>\n          <\/template>\n          <template #accordion-content>\n            <h4><strong>Jennifer Hernandez, M.S.<br \/>\n<\/strong>Research Associate<br \/>\n<a href=\"mailto:jenniferhernandez@westernu.edu\">jenniferhernandez@westernu.edu<\/a><\/h4>\n<p>Jennifer Hernandez received her Bachelor of Science degree in Biology from California State University, Los Angeles in 2016. She then earned a Masters of Science degree in Biomedical Sciences from Western University of Health Sciences in 2018. She did her master\u2019s thesis work under the supervision of Dr. Ed Wagner at Western University of Health Sciences and was hired as a full time research associate for the same lab in the summer of 2018. During this time she became proficient in electrophysiological recordings from a particular subset of neurons involved in the hypothalamic feeding circuity. She was also instrumental in establishing colonies of transgenic mice including eGFP-POMC, NR5A1-Cre, eGFP-POMC\/NR5A1-Cre, and PACAP-Cre. She not only established the colonies, but has successfully implemented and developed the genotyping procedures for these different strains. During her research, she has also become proficient at different surgical procedures such as stereotaxic viral injections and cannulations, as well as ovariectomies. She has also become well versed in <em>iv vivo<\/em> feeding and metabolic studies for both mice as well as guinea pig models and has performed numerous <em>in vitro <\/em>studies involving the aforementioned strains. These different procedures have expanded her knowledge of the hypothalamic feeding circuitry, as well as the sex differences and dietary differences that affect energy balance. She has also played a role in mentoring and teaching various masters, medical, veterinary, and high school students during her time at Western University.<\/p>\n<h4><\/h4>\n<h4><strong>Cassandra Gastelum<\/strong><br \/>\nProject Specialist\/ MSBS Graduate Student C\/O 2021<br \/>\n<a href=\"mailto:cassandra.gastelum@westernu.edu\">cassandra.gastelum@westernu.edu<\/a><\/h4>\n<p>&nbsp;<\/p>\n<h4><strong>Nikki Le<\/strong><br \/>\nProject Specialist\/ MSBS Graduate Student C\/O 2021<br \/>\n<a href=\"mailto:nikki.le@westernu.edu\">nikki.le@westernu.edu<\/a><\/h4>\n\n          <\/template>\n        <\/app-accordion-item>\n          <\/app-accordion>\n  <\/div>\n<\/section>\n\n<section class=\"faculty-card my-48 md:my-72\">\n  <div class=\"container\">\n        <div class=\"md:flex md:justify-between md:items-end mb-20\">\n      <h2 class=\"t-h2-sans  text-red md:mr-40\">ED WAGNER, PhD<\/h2>\n    <\/div>\n            <div class=\"md:flex md:flex-wrap px-12\">\n                                                      \n                                                        \n      <div class=\"px-12 mt-24 w-full md:w-1\/2\">\n        <div class=\"sm:flex w-full py-32 border-b-1 border-gray\">\n                                            <a href=\"\/bios\/?bio=ewagner\" class=\"card block w-256 sm:w-1\/3 pl-12 md:pl-0 pr-12 md:pr-18 mr-auto md:mr-0\">\n                <div class=\"rounded-5 md:rounded-8 overflow-hidden\">\n                    <img decoding=\"async\" class=\"card__image w-full h-256 sm:h-192 md:h-144 lg:h-192 xl:h-256 object-cover\" alt=\"Portrait of ED WAGNER, PhD\" src=\"\/media\/westernu\/images\/staff\/ewagner.jpg\" \/>\n                <\/div>\n            <\/a>\n                                <div class=\"w-full h-full md:w-2\/3 pl-12 md:pl-18 mt-18 sm:mt-0\">\n            <p class=\"t-h2-serif leading-120\">\n                            <a href=\"\/bios\/?bio=ewagner\" class=\"text-red-light hover:text-red\">ED WAGNER, PhD<\/a>\n                          <\/p>\n            <div class=\"mt-16 t-p2\">\n                            <p class=\"font-bold\">College of Osteopathic Medicine of the Pacific<\/p>\n                                          <p class=\"font-bold\">Professor of Physiology<\/p>\n                                          <p>\n                <a href=\"tel:909-469-5239\" class=\"hover:underline\">909-469-5239<\/a>\n              <\/p>\n                                                        <p>\n                <a href=\"mailto:ewagner@westernu.edu\" class=\"hover:underline\">ewagner@westernu.edu <\/a>\n              <\/p>\n                                        <\/div>\n          <\/div>\n        <\/div>\n      <\/div>\n          <\/div>\n      <\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":80,"featured_media":0,"parent":8215,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-4321","page","type-page","status-publish","hentry"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages\/4321","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/users\/80"}],"replies":[{"embeddable":true,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/comments?post=4321"}],"version-history":[{"count":15,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages\/4321\/revisions"}],"predecessor-version":[{"id":14846,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages\/4321\/revisions\/14846"}],"up":[{"embeddable":true,"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/pages\/8215"}],"wp:attachment":[{"href":"https:\/\/stagewp.westernu.edu\/osteopathic\/wp-json\/wp\/v2\/media?parent=4321"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}